{"created":"2023-05-15T08:55:28.162572+00:00","id":13704,"links":{},"metadata":{"_buckets":{"deposit":"7f9444b4-b1e2-4e76-8a26-f1e5caa8804e"},"_deposit":{"created_by":3,"id":"13704","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13704"},"status":"published"},"_oai":{"id":"oai:uuair.repo.nii.ac.jp:00013704","sets":["716:720:887"]},"author_link":["22553","22554","22555","22556","22557","22558"],"control_number":"13704","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"145","bibliographicPageStart":"136","bibliographicVolumeNumber":"193","bibliographic_titles":[{"bibliographic_title":"Theriogenology","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_10001_description_24":{"attribute_name":"WEKOアイテムタイプ","attribute_value_mlt":[{"subitem_description":"学術雑誌論文 / Journal Article","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The cumulus oophorus is a structure that surrounds the mammalian egg and plays a key role in fertilization. However, very little is known with regards to how secretions from the cumulus cells can specifically promote fertilization. We hypothesized that secretions from bovine cumulus cells, and the reduction of oxygen stress by metabolic change, would enhance the fertilization capacity of sperm during in vitro fertilization (IVF) procedures. To prove our hypothesis, sperm were pre-incubated in chemically defined capacitation media containing methyl-beta-cyclodextrin and used to inseminate cumulus cell oocyte complexes, or denuded oocytes, with some components. While sperm capacitation was induced in capacitation media, fertilization was impeded by the removal of cumulus cells from cumulus cell oocyte complexes. Secretions from cumulus cells promoted the formation of two pronuclei via a filter and the fertilization of denuded oocytes was dramatically enhanced with hyaluronate, low oxygen concentration, or progesterone in fertilization media (P < 0.05). This demonstrates that these factors-maintained sperm motility and capacitation or enhanced the hyper-activation of capacitated sperm (P < 0.05). We conclude that cumulus cells secrete progesterone, hyaluronate and undergo metabolic events to reduce oxidative stress in fertilization media. These phenomenons help to improve the fertilization capacity of sperm. We believe that this study makes a significant contribution to our understanding of the function of cumulus cells during fertilization in animals and humans.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.theriogenology.2022.08.025","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Elsevier Publisher Edition"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0093691X22003430","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0093-691X","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 翼","creatorNameLang":"ja"},{"creatorName":"カトウ, ヨク","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Yoku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"22553","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohshima, Yuki","creatorNameLang":"en"},{"creatorName":"オオシマ, ユキ","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"22554","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Aiko","creatorNameLang":"en"},{"creatorName":"ササキ, アイコ","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"22555","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshikawa, Eri","creatorNameLang":"en"},{"creatorName":"ヨシカワ, エリ","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"22556","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Xu, Hua","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"22557","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長尾, 慶和","creatorNameLang":"ja"},{"creatorName":"ナガオ, ヨシカズ","creatorNameLang":"ja-Kana"},{"creatorName":"Nagao, Yoshikazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"22558","nameIdentifierScheme":"WEKO"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"積雲セル","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"受胎","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"精子の受精能獲得","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"分泌物","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"活性酸素種","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"プロゲステロン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒアルロン酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Cumulus cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fertilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sperm capacitation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Secretions","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Reactive oxygen species","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Progesterone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hyaluronate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The secretion and metabolism of cumulus cells support fertilization in the bovine model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The secretion and metabolism of cumulus cells support fertilization in the bovine model","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["887"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-28"},"publish_date":"2023-03-28","publish_status":"0","recid":"13704","relation_version_is_last":true,"title":["The secretion and metabolism of cumulus cells support fertilization in the bovine model"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-07-10T05:23:32.841302+00:00"}